COMPOUNDS, NUTRIENT ANALYSIS,ANTIOXIDANT, FLAVONOIDS. POLYPHENOLS,COSMETIC,COLOURANT,TABLE OF APIGENINIDIN ETC. IN JOBELYN
“Let medicine be thy food and let food be thy medicine.” — Hippocrates
Could Jobelyn be regarded as the super food of the modern times?
Our research studies on Jobelyn in the last ten years had been concentrated on establishing the medicinal values of the product and several articles have been published to show that:
Its Health benefits include:
- Multi-facetted anti-inflammatory properties
- Immune activation
- Hematopoietic effects
- Pain reduction
Ref: (1) Clinical Efficacy of a West African Sorghum bicolor-Based Traditional Herbal Preparation Jobelyn Shows Increased Hemoglobin and CD4+ T-Lymphocyte Counts in HIV-Positive Patients.
J Altern Complement Med. Jan 1, 2014; 20(1): 53–56.doi: 10.1089/acm.2013.0125
(2) West African Sorghum bicolor Leaf Sheaths Have Anti-Inflammatory and Immune-Modulating Properties In Vitro – Published in JOURNAL OF MEDICINAL FOOD J Med Food 00 (0) 2013, 1–9
With the renewed interest in the effect of Nutrition on health, Health Forever Product Limited had commissioned the GMP Laboratory of California to do a comprehensive Nutritional Facts Analysis of Jobelyn. Jobelyn is packaged from Sorghum bicolor leaf sheaths as nature made it and so it is 100% natural without any additive. The result could be accessed at:
http://www.afritradomedic.com/pdf/Jobelyn%20Nutritional%20Facts%20Analysis%20%20GMP%20Labs.pdf
It showed that Jobelyn is not only rich in food such as Carbohydrates, Protein and Dietary Fibre but also in Vitamins, Elements and Fatty Acids.
It could be described as a perfect fit for the famous Hippocrates quote “Let food be thy medicine and medicine be thy food.”
Our discovery about Jobelyn’s nutritional properties is very timely as a recent release about concerns of taking supplements would create a major concern for millions of people who are trying to maintain wellness by consuming dietary supplements. Please see the link below for the publication.
https://www.yahoo.com/health/dietary-supplements-linked-to-increased-cancer-117012426432.html
A large-scale study has shown that over-supplementation could be very bad for our health.
Could it be possible that too many vitamins and minerals can bring about various forms of cancer? Yes, according to a large-scale study delivered during the American Association for Cancer Research (AACR) Annual Meeting 2015.
The research presented at this conference dates back 20 years, when scientists discovered that a diet rich in fruits and vegetables was shown to keep cancer at bay. Then study experts shifted their focus to over-the-counter supplements, assuming that consuming extra vitamins and minerals would also produce anti-cancer benefits.
First came the testing on animals and the results were encouraging. Scientists then studied thousands of patients—over 300,000—who were given either dietary supplements or a placebo over a 10-year period.
And their findings were alarming.
“We show that not only is supplementing your diet with vitamins that are found in a healthy diet unlikely to decrease your risk of developing cancer, but in some cases, taking more than the recommended daily allowance of these supplements can increase cancer risk,” lead investigator Tim Byers, MD, MPH from the University of Colorado Cancer Center tells Yahoo Health. “More specifically, taking more than the recommended daily allowance of folic acid, Vitamin E and beta-carotene were all shown to increase cancer risk.”
CONTENTS OF JOBELYN
NOVEL COMPOUNDS
| Sample | Structure | Name | lmax (nm) | [M+H]+ (m/z) | Major fragments
[M+H]+ |
Amount in leaf sheath powder (mg/g) |
| P8 | Apigenin | 335 | 271 | – | 29.87±9.85 | |
| 7-methoxyflavone-apigeninidin adduct (Apigeninidin-7-O-methylflavene dimmer) | 479 | 523 | 384, 269, 255 | 2.80±0.29 | ||
| P9 | Flavone-apigeninidin adduct (Apigeninidin-flavene dimmer) | 480 | 509 | 384, 255 | 7.70±0.65 |
Geera B, Ojwang LO, Awika JM. New highly stable dimeric 3-deoxyanthocyanidin pigments from sorghum bicolor leaf sheath. J Food Sci. 2012 May;77(5):C566-72. doi: 10.1111/j.1750-3841.2012.02668.x. Epub 2012 Apr 10. PubMed PMID: 22489620
TABLE OF ANTIOXIDANT COMPOUNDS IN JOBELYN
| Lot No | BL ID No. | Apigeninidin | Luteolinindin | Apigenin | Luteolin | Naringenin |
| 12-2003 | 06-4107 | 14,650 | 216 | 3,695 | 530 | 151 |
| 12-2005 | 06-4108 | 23,950 | 328 | 5,100 | 715 | 244 |
| 12-2006 RAT 1:5 | 06-4109 | 22,950 | 229 | 3,770 | 396 | 90.0 |
| 12-2006 RAT 1:10 | 06-4110 | 27,650 | 286 | 4,770 | 570 | 122 |
| 12-2006 RAT 1:20 | 06-4111 | 31,900 | 331 | 5,300 | 640 | 149 |
| 12-2006 New Pot | 06-4112 | 21,850 | 168 | 2,975 | 218 | 71.0 |
| 12-2005 RAT 1:20 | 06-4812 | 38,650 | 323 | 6,020 | 585 | 230 |
| 12-2006 RAT 1:50 | 06-4813 | 29,850 | 170 | 4,050 | 339 | 118 |
| lot#11 | 08-3552 | 39,900 | 450 | 6,910 | 570 | 130 |
| lot# 54FBT99 | 09-0590 | 47,380 | 890 | 6,460 | 673 | 166 |
Ayuba GI, Jensen GS, Benson KF, Okubena AM, Okubena O. Clinical efficacy of a West African sorghum bicolor-based traditional herbal preparation Jobelyn shows increased hemoglobin and CD4+ T-lymphocyte counts in HIV-positive patients. J Altern Complement Med. 2014 Jan;20(1):53-6. doi: 10.1089/acm.2013.0125. Epub 2013 Nov 27. PubMed PMID: 24283768
TOTAL BIOFLAVONOIDS IN JOBELYN
Sample ID Brunswick ORAChydro* Total bioflavonoids ¹
Lab ID (mmoleTE/g) (mg/g)
Jobelyn sample 08-3552 2,156 254.27
Lot#11
*The ORAC analysis provides a measure of the scavenging capacity of antioxidants against the peroxyl radical,
which is one of the most common reactive oxygen species (ROS) found in the body. ORAChydro reflects water-soluble
antioxidant capacity. Trolox, a water-soluble Vitamin E analog, is used as the calibration standard and the ORAC
result is expressed as micromole Trolox equivalent (TE) per gram.
¹ The total bioflavonoids result is expressed as milligram rutin equivalent per gram.
The acceptable precision of the ORAC assay is 15% relative standard deviation.[1]–[2]
Testing performed by Y. Kou & H. Ji.
Approved by:______________
Boxin Ou, Ph.D.
B-8372/ Y. Kou 12-15-08, revised mk 12-16-08
Samples will be discarded one month from report date, unless otherwise notified by customer in writing.
PHENOLICS IN JOBELYN
Sample ID Brunswick ORAChydro* Phenolics ¹
Lab ID (mmoleTE/g) (mg/g)
Jobelyn Sample 06-3017 3,123 106.86
Jobelyn 21/07/2006
*The ORAC analysis provides a measure of the scavenging capacity of antioxidants against the peroxyl
radical, which is one of the most common reactive oxygen species (ROS) found in the body. ORAChydro
reflects water-soluble antioxidant capacity.
Trolox, a water-soluble Vitamin E analog, is used as the calibration standard and the ORAC
result is expressed as micromole Trolox equivalent (TE) per gram.
¹ The phenolic result is expressed as milligram gallic acid equivalent per gram.
The acceptable precision of the ORAC assay is 15% relative standard deviation.[3]
ANTIOXIDANTS IN JOBELYN
06/27/2011 Page 1 of 1
Certificate of Analysis
Customer: Health Forever Product Limited
Sample Identification:
Batch #: B-11452
BL ID #: 11-1161
Description: Jobelyn, Powder
Date Received: 06/15/2011
| Custom Manufacturing & Formulations |
| 2931 E. La Jolla Street, Anaheim, CA 92806 – TEL: (714) 630-2467 – FAX: (714) 237-1374 |
| QUALITY CONTROL TEST REPORT NUTRITIONAL ANALYSIS |
| Custom Manufacturing & Formulations |
| 2931 E. La Jolla Street, Anaheim, CA 92806 – TEL: (714) 630-2467 – FAX: (714) 237-1374 |
| Custom Manufacturing & Formulations |
| 2931 E. La Jolla Street, Anaheim, CA 92806 – TEL: (714) 630-2467 – FAX: (714) 237-1374 |
| (QC Manager) |
STUDY ON JOBELYN AS FOOD COLORANT.
Thermal stability of 3-deoxyanthocyanidin pigments (JOBELYN)
3-Deoxyanthocyanidins are promising natural colourants due to their unique properties compared to anthocyanins. However, thermal stability of 3-deoxyanthocyanidins is largely unknown. Thermal stability of crude and pure 3-deoxyanthocyanidins was determined at 95 _C/2 h and 121 _C/30 min, at pH 1–7 using HCl, formic or citric acid as acidulants. The colour retention of crude and pure 3-deoxyanthocyanidins (79–89% after 95 _C/2 h and 39–118% after 121 _C/30 min) was high compared to literature reports for anthocyanins under similar treatments. pH significantly affected the thermal stability of 3-deoxyanthocyanidins:
Colour retention was better at pH 1–2 (70.2–118%) than at pH 3–7 (39.0–86.8%). Chalcones were identified as the major heat degradation products at pH 3–7. Slow rate of chalcone formation and resistance to C-ring fission were identified as the major contributors to thermal stability of 3-deoxyanthocyanidins.
Overall, the heat stability of 3-deoxyanthocyanidins indicates good potential for food use.
2.1.1. Crude 3-deoxyanthocyanidin pigments
Crude 3-deoxyanthocyanidin extract was obtained from dried red sorghum leaf sheaths (Health Forever Products, Lagos, Nigeria). This material was previously described by Geera, Ojwang, and Awika (2012). Acidified aqueous methanol (formic acid:water:methanol = 1:49:50, v:v:v) was used to extract the sample, with 2 h shaking at room temperature. Solvent was removed to complete dryness under vacuum at 40 _C using a Multivapor system (Büchi, Flawil, Switzland).
- Conclusion
Both crude 3-deoxyanthocyanidin extract and pure 3-deoxyanthocyanidins showed good thermal stability after heat treatments at 95 _C/2 h and 121 _C/30 min. The pure 3-deoxyanthocyanidins were particularly stable over a broad range of pH, which suggests good potential as natural colourants. The crude 3-deoxyanthocyanidin extract tend to be less stable than the pure 3-deoxyanthocyanidins at 121 _C/30 min. This suggests that other phenolic constituents in the crude extract could affect thermal stability of the pigments, especially at temperatures which degradation of polyphenols could occur. More studies are needed to establish the effect of 3-deoxyanthocyanidin and copigment composition of crude pigment extract on thermal stability of sorghum 3-deoxyanthocyanidins. The type of acid affected thermal stability of 3-deoxyanthocyanidins, and this indicates characteristics of acidulants should be taken into considerations when processing products containing 3-deoxyanthocyanidin pigments. Molecular structure could also affect thermal stability of 3-deoxyanthocyanidins under certain conditions. For example, compared with non-methoxylated 3-deoxyanthocyanidins, methoxylated ones had reduced thermal stability when treated in pH 1 and 2 formic acid solutions. This suggests the composition of 3-deoxyanthocyanidin pigments should also be considered when utilizing these pigments in products. Overall, this study showed that 3-deoxyanthocyanidins possess good thermal stability, even at neutral and low acidic conditions (pH 4–6). This property makes 3-deoxyanthocyanidinsNsuitable to be utilized as natural food colourants in various food and beverage products of low acidity.
Thermal stability of 3-deoxyanthocyanidin pigments Liyi Yang , Linda Dykes , Joseph M. Awika Journal of Food Chemistry http://dx.doi.org/10.1016/j.foodchem.2014.03.105
COSMECUTICAL POTENTIALS OF JOBELYN FROM BRUNSWICK LABS USA
Assays Performed Results IC50
Collagenase Inhibition1 60 µg/mL
Elastase inhibition1 17 µg/mL
Anti-glycation2 4 µg/mL
Comments:
- Wrinkle is a product of aging, smoking, sun exposition, pollution and etc. With the decrement of collagen (which provides skin firmness) and elastin (which supplies skin elasticity and rebound), appearances of roughness, uneven tone, brown patches, thin skin and deep wrinkles will show up. A product’s anti-wrinkle activity can be determined by its inhibitory capacity on collagenase or elastase. Jobelyn was analyzed against common benchmarks that are known for anti-wrinkling.
Collagenase inhibition: 15-fold potency of Vitamin C; 8-fold potency of Idebenone; 30-fold potency of ferrulic acid.
Elastase inhibition: 22-fold potency of Vitamin C; 8-fold potency of ferrulic acid; 1.5-fold potency of quercetin.
- Collagens are important proteins for the skin, as they are essential for structure and function of the extracellular matrix of the dermis. Thinner and wrinkled skin, the typical signs of normal aging, are the consequences of reduced collagen. Protein glycation contributes to skin aging as it deteriorates the existing collagen by crosslinking. Accelerated skin aging is especially noticeable in diabetic patients, where glycation is increased because of the high serum glucose level. Jobelyn was analyzed in parallel with ascorbic acid and alpha-tocophenol (chose1benchmarks), neither ascorbic acid nor alpha-tocophenol showed significant anti-glycation activity.
Reference:
Demeule M, Brossard M, Page M, GingrasD., Beliveau R. Matrix metalloproteinase inhibition by green tea catechins. Biochim. Biophys. Acta. 1478: 51-60, 2000
Generation of Active Oxygen Species from Advanced Glycation End-Products (AGE) under Ultraviolet Light A (UVA) Irradiation Hitoshi Masaki, Yuri Okano and Hiromu Sakurai, Biochemical and Biophysical Research Communications 235 (2) 1997, 306-310.
REFERENCES FOR STUDIES DONE ON JOBELYN
Ayuba GI, Jensen GS, Benson KF, Okubena AM, Okubena O. Clinical efficacy of a West African sorghum bicolor-based traditional herbal preparation Jobelyn shows increased hemoglobin and CD4+ T-lymphocyte counts in HIV-positive patients. J Altern Complement Med. 2014 Jan;20(1):53-6. doi: 10.1089/acm.2013.0125. Epub 2013 Nov 27. PubMed PMID: 24283768
Geera B, Ojwang LO, Awika JM. New highly stable dimeric 3-deoxyanthocyanidin pigments from sorghum bicolor leaf sheath. J Food Sci. 2012 May;77(5):C566-72. doi: 10.1111/j.1750-3841.2012.02668.x. Epub 2012 Apr 10. PubMed PMID: 22489620
Benson KF, Beaman JL, Ou B, Okubena A, Okubena O, Jensen GS. West African Sorghum bicolor leaf sheaths have anti-inflammatory and immune-modulating properties in vitro. J Med Food. 2013 Mar;16(3):230-8. doi: 10.1089/jmf.2012.0214. Epub 2013 Jan 5. PubMed PMID: 23289787
Umukoro S, Adrian Omogbiya I, Taghogho Eduviere A. Evaluation of the Effect of Jobelyn® on Chemoconvulsants- Induced Seizure in Mice. BCN. 2013; 4 (2) 4 (2) :19-23 URL http://bcn.iums.ac.ir/browse.php?a_code=A-10-151&slc_lang=en&sid=1
Umukoro S, Ugbomah A, Aderibigbe A, Eduviere A, Omogbiya A. Antioxidant Property of Jobelyn as the Possible Mechanism Underlying its Anti-amnesic Activity in Rodents. BCN. 2013; 4 (1) 4 (1) :42-49 URL http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-139&slc_lang=en&sid=1
Omogbiya IA, Umukoro S, Aderibigbe AO, Bakre AG. Jobelyn® pretreatment ameliorates symptoms of psychosis in experimental models. J Basic Clin Physiol Pharmacol. 2013;24(4):331-6. doi: 10.1515/jbcpp-2012-0073. PubMed PMID: 23412872
Umukoro S, Omogbiya IA, Eduviere TA. Effect of Jobelyn® on intruder- and isolation-induced aggressive behavior in mice. J Basic Clin Physiol Pharmacol. 2013;24(4):263-9. doi: 10.1515/jbcpp-2012-0069. PubMed PMID: 23729562
Toxicological Profiles of Commercial Herbal Preparation, Jobelyn® nternational Journal of Health Research, December 2009; 2(4): 369-374 © Poracom Academic Publishers. All rights reserved. Available at http://www.ijhr.org
The influence of African Herbal Formula on the haematological parameters of trypanosome infected rats VI Okochi, J Okpuzor, MO Okubena, AK Awoyemi African Journal of Biotechnology Vol.2(9) 2003: 312-316
Response of Trypanosoma brucei brucei-induced anaemia to a commercial herbal preparation ……….. 4 African Journal of Biotechnology Vol. 2 (9), pp. 307‐311, September 2003
Thermal stability of 3-deoxyanthocyanidin pigments Liyi Yang , Linda Dykes , Joseph M. Awika Journal of Food Chemistry http://dx.doi.org/10.1016/j.foodchem.2014.03.105
